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rabbit polyclonal anti neu  (Proteintech)


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    Proteintech rabbit polyclonal anti neu
    Rabbit Polyclonal Anti Neu, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 604 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+neu/pmc12271594-5-0-4?v=Proteintech
    Average 96 stars, based on 604 article reviews
    rabbit polyclonal anti neu - by Bioz Stars, 2026-07
    96/100 stars

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    Figure 3. Endothelial Nrg1 expression and cardiac ErbB signaling are suppressed in Bmx/CA mice. (a) Quantitative RT-PCR analysis of Nrg1 expression in cardiac ECs isolated from Bmx/CA mice (Bmx/CA ECs) compared with ECs isolated from Ctrl mice (Ctrl ECs). **P < 0.01 (n = 4). (b) Quantitative RT-PCR analysis of Nrg1 and Axin2 expression in HCAECs stimulated with recombinant human Wnt3a (200 ng/mL) (Wnt-3a) or PBS containing 0.1% BSA (Ctrl). **P < 0.01, *P < 0.05 (n = 4). (c) Immunoprecipitation and western blotting analysis of <t>ErbB2</t> and ErbB4 phosphorylation. Lysates from the heart of Ctrl or Bmx/CA mice at 1 week after TAM were precipitated with anti-ErbB2 or anti-ErbB4 antibody and the precipitates were blotted with anti- phosphorylated tyrosine antibody (pTyr (PY99)) or with the antibody used for precipitation. Phosphorylation levels were analyzed from the band density using ImageJ (Bottom panel). *P < 0.05 (n = 4).
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    Figure 3. Endothelial Nrg1 expression and cardiac ErbB signaling are suppressed in Bmx/CA mice. (a) Quantitative RT-PCR analysis of Nrg1 expression in cardiac ECs isolated from Bmx/CA mice (Bmx/CA ECs) compared with ECs isolated from Ctrl mice (Ctrl ECs). **P < 0.01 (n = 4). (b) Quantitative RT-PCR analysis of Nrg1 and Axin2 expression in HCAECs stimulated with recombinant human Wnt3a (200 ng/mL) (Wnt-3a) or PBS containing 0.1% BSA (Ctrl). **P < 0.01, *P < 0.05 (n = 4). (c) Immunoprecipitation and western blotting analysis of ErbB2 and ErbB4 phosphorylation. Lysates from the heart of Ctrl or Bmx/CA mice at 1 week after TAM were precipitated with anti-ErbB2 or anti-ErbB4 antibody and the precipitates were blotted with anti- phosphorylated tyrosine antibody (pTyr (PY99)) or with the antibody used for precipitation. Phosphorylation levels were analyzed from the band density using ImageJ (Bottom panel). *P < 0.05 (n = 4).

    Journal: Scientific reports

    Article Title: Activation of endothelial β-catenin signaling induces heart failure.

    doi: 10.1038/srep25009

    Figure Lengend Snippet: Figure 3. Endothelial Nrg1 expression and cardiac ErbB signaling are suppressed in Bmx/CA mice. (a) Quantitative RT-PCR analysis of Nrg1 expression in cardiac ECs isolated from Bmx/CA mice (Bmx/CA ECs) compared with ECs isolated from Ctrl mice (Ctrl ECs). **P < 0.01 (n = 4). (b) Quantitative RT-PCR analysis of Nrg1 and Axin2 expression in HCAECs stimulated with recombinant human Wnt3a (200 ng/mL) (Wnt-3a) or PBS containing 0.1% BSA (Ctrl). **P < 0.01, *P < 0.05 (n = 4). (c) Immunoprecipitation and western blotting analysis of ErbB2 and ErbB4 phosphorylation. Lysates from the heart of Ctrl or Bmx/CA mice at 1 week after TAM were precipitated with anti-ErbB2 or anti-ErbB4 antibody and the precipitates were blotted with anti- phosphorylated tyrosine antibody (pTyr (PY99)) or with the antibody used for precipitation. Phosphorylation levels were analyzed from the band density using ImageJ (Bottom panel). *P < 0.05 (n = 4).

    Article Snippet: Rabbit polyclonal antibody against ErbB2 (Neu) (C-18) (sc-284), rabbit polyclonal antibody against ErbB4 (C-18) (sc-283) and mouse monoclonal antibody against phosphorylated tyrosine (PY99) (sc-7020) were from Santa Cruz Biotechnology.

    Techniques: Expressing, Quantitative RT-PCR, Isolation, Recombinant, Immunoprecipitation, Western Blot, Phospho-proteomics

    Figure 4. Administration of NRG1 rescued the cardiac phenotypes of Bmx/CA mice. (a) Immunoprecipitation and western blotting analyses of ErbB2 and ErbB4 phosphorylation. Lysates from the heart of Bmx/CA treated with or without rNRG were precipitated with anti-ErbB2 or anti-ErbB4 antibody and the precipitates were blotted with anti-phosphorylated tyrosine antibody (pTyr (PY99)) or with the antibody used for precipitation. Phosphorylation levels were analyzed from the band density using ImageJ (Right panel). *P < 0.05 (n = 4). (b) Cardiac function of Ctrl mice (Ctrl) and Bmx/CA mice treated with vehicle (Bmx/CA) or rNRG (Bmx/CA + rNRG) were evaluated by echocardiography before (Pre), 3rd and 5th day of TAM treatment (Tam3 and Tam5), and at the indicated time point after the 5 day- TAM treatment. **P < 0.01 versus Ctrl, ††P < 0.01 versus Bmx/CA + rNRG, §P < 0.05 versus Ctrl. (c) Representative electron microscopic images of the heart tissue from Ctrl and Bmx/CA mice treated with vehicle (Bmx/CA) or rNRG (Bmx/CA + rNRG). Scale bar: 2.5 μ m. (d) Quantitation of vacuole formations. Vacuoles were evaluated as described in Fig. 2i. **P < 0.01.

    Journal: Scientific reports

    Article Title: Activation of endothelial β-catenin signaling induces heart failure.

    doi: 10.1038/srep25009

    Figure Lengend Snippet: Figure 4. Administration of NRG1 rescued the cardiac phenotypes of Bmx/CA mice. (a) Immunoprecipitation and western blotting analyses of ErbB2 and ErbB4 phosphorylation. Lysates from the heart of Bmx/CA treated with or without rNRG were precipitated with anti-ErbB2 or anti-ErbB4 antibody and the precipitates were blotted with anti-phosphorylated tyrosine antibody (pTyr (PY99)) or with the antibody used for precipitation. Phosphorylation levels were analyzed from the band density using ImageJ (Right panel). *P < 0.05 (n = 4). (b) Cardiac function of Ctrl mice (Ctrl) and Bmx/CA mice treated with vehicle (Bmx/CA) or rNRG (Bmx/CA + rNRG) were evaluated by echocardiography before (Pre), 3rd and 5th day of TAM treatment (Tam3 and Tam5), and at the indicated time point after the 5 day- TAM treatment. **P < 0.01 versus Ctrl, ††P < 0.01 versus Bmx/CA + rNRG, §P < 0.05 versus Ctrl. (c) Representative electron microscopic images of the heart tissue from Ctrl and Bmx/CA mice treated with vehicle (Bmx/CA) or rNRG (Bmx/CA + rNRG). Scale bar: 2.5 μ m. (d) Quantitation of vacuole formations. Vacuoles were evaluated as described in Fig. 2i. **P < 0.01.

    Article Snippet: Rabbit polyclonal antibody against ErbB2 (Neu) (C-18) (sc-284), rabbit polyclonal antibody against ErbB4 (C-18) (sc-283) and mouse monoclonal antibody against phosphorylated tyrosine (PY99) (sc-7020) were from Santa Cruz Biotechnology.

    Techniques: Immunoprecipitation, Western Blot, Phospho-proteomics, Quantitation Assay